Invitrogen TNF alpha Bovine ELISA Kit, 96 Tests

Invitrogen TNF alpha Bovine ELISA Kit, 96 Tests

The Bovine Tumor Necrosis Factor alpha (Bov TNFα) ELISA quantitates Bov TNFα in bovine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Bov TNFα.
Principle of the method
The Bovine TNFα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: <12%
• Intraassay CV: <10%

Contents    

• Bovine TNF-a Antibody Coated wells, 96-well plate, 1 plate
• Bovine TNF-a Biotin Conjugate, 2 vials
• Bovine TNF-a Standard, recombinant bovine TNF-a, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent A (contains 0.09% sodium azide), 30 mL
• Assay Diluent B (5X), 15 mL
• Streptavidin-HRP (600X), 0.2 mL
• TMB Substrate, 12 mL
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2